LOWER NON-SPECIFIC ADSORPTION
WITH AFFICOAT

The AffiCoat forms a protective self-assembled monolayer on metallic materials that significantly improves molecular recognition by minimizing non-specific adsorption. The proprietary heteropeptide sequence of AffiCoat has proven to be better than other broadly used anti-fouling chemistry such as Dextran, PEG-COOH and alkanethiols. 

KEY BENEFITS INCLUDE:
  • Low biofouling
  • Increase activity of immobilized receptor
  • Two-step overnight preparation
  • Shelf-stable protective film on a surface
  • EDC-NHS chemistry compatibility
  • Compatibility with assays developed for CM Dextran and PEG 

TWO-STEP PREPARATION

  1. Immersed your SPR sensor or other surface of interest in 5 mM peptide solution in absolute ethanol for at least 16 hours to form a well-ordered monolayer.

  2. Wash, dry with nitrogen and store in a dry place. Stable for at least (six) 6 months.
KEY LITERATURE
  • Bolduc OR et al., SPR Biosensing in Crude Serum Using Ultralow Fouling Mixed Peptide SAM, Analytical Chem 2010, 82, 3699-3706.
  • Bolduc OR et al., Peptide Self-Assembled Monolayers for Label-Free and Unamplified SPR Biosensing in Crude Cell Lysate, Analytical Chem 2009, 81, 6779-6788.
  • Bolduc OR et al., Monolayers of 3-Mercaptopropyl – Amino Acid to Reduce the Non-Specific Adsorption of Serum Proteins on the Surface of Biosensors, Langmuir 2008, 24, 12085-12091