Resources
Resources
Tech notes to understand SPR, guides to read your data, protocols to run the assay, and application notes that show it done.
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Sensors & kitsFind the right sensorThree questions to the sensor, the kit and the protocol to run it.Open →GuideRead your sensorgramWhat each phase means, and what to do when it looks wrong.Open →GuideStatic or kinetic SPR?P4SPR 2.0 or P4PRO with AffiPump: which measurement your question needs.Open →Tech note 1What SPR measuresThe first of ten tech notes, from light on gold to a trustworthy answer.Open →
Tech notes
Ten notes that follow the measurement chain, from what SPR measures to a trustworthy answer.
TN-01What SPR measuresSurface, dip, sensorgram5 min
TN-02Why a gold filmPlasmon, detection zone, film vs particles6 min
TN-03Lensless optics and wavelength readoutReadouts, lensless design7 min
TN-04Where the precision comes fromReadouts, dip tracking, resolution6 min
TN-05The chipGold film, film quality, handling7 min
TN-06The binding surfaceLinkers, coupling, regeneration8 min
TN-07Designing a binding experimentSurface partner, concentrations, controls7 min
TN-08Delivering the sample: static and flowDiffusion, flow, mass transport6 min
TN-09Getting a clean signalReferencing, buffer match, drift6 min
TN-10From sensorgram to answerAffinity, kinetics, residuals7 minGuides
GuideDecoding SPR Sensorgrams14 curve shapes, causes and fixes
GuideSensors & kitsThree questions to the right sensor, its kit and the protocol to run it. Or browse all of them, add what you need to a l
GuideSPR Sensorgram ExplainedHow to read an SPR sensorgram phase by phase, extract ka, kd and KD from the curve, and spot what baseline drift or inco
GuideStatic vs Kinetic SPRWhen to use each mode, what data you get, and how they compare: static on the P4SPR 2.0, kinetic on the P4PRO with AffiPPDF
GuideSPR Regeneration GuideGuide · on the current site ↗
GuideHow Does Surface Plasmon Resonance Work?Article · on the current site ↗Protocols
#1Covalent Immobilization of ProteinsP4SPR#2Metal Chelation Immobilization of His-Tagged ProteinsP4SPR#3Online Streptavidin Capture of ProteinsP4SPR#4P4SPR 2.0 Calibration Using NaCl SolutionsP4SPR 2.0#5Starter Test Kit: Covalent Immobilization & Ligand Binding AssayP4SPR 2.0Application notes
App noteSPR for AAV Bioproduction in Gene TherapyP4SPR, P4PRO · AAV Bioproduction / Gene Therapy · AAV Total Capsid TiterPDF
App noteMeasuring a Therapeutic Antibody's Binding to Plasma ProteinsP4SPR · Antibody characterization, drug development
App noteRapid Quality Control of Antibodies Using SPRP4SPR · Antibody Quality Control · SARS-CoV-2 Nucleocapsid ProteinPDF
App noteAssay Optimization of Protein–Protein InteractionP4SPR · Immunosensing · Anti-EcAII antibody
App noteOn-site Testing of RDX in Environmental WatersP4SPR · Small Molecule DetectionPDF
App noteGene Regulation, Lac Operon / Lac RepressorP4SPR · DNA–Protein BindingPDF
App noteGlycan and Lectin Assays on the P4SPRP4SPR · Glycobiology, cancer serology, bacterial identification
App noteScreening Competitors of Heparin-Binding ProteinsP4SPR · Glycobiology, inhibitor screening
App noteScreening Plasma for IgA Deficiency in Under an HourP4SPR · Clinical serology, blood banking
App noteMeasuring Neutralizing Antibodies in SerumP4SPR · Serology, vaccine and immunity studies
App noteAffinité's P4PRO: Precision Binding Kinetics Made SimpleP4PRO · Binding KineticsPDF
App noteRapid Screening of Protein–Protein InteractionsP4SPR · Protein–Protein InteractionsPDF
App noteChecking the Activity of Protein Reference MaterialsP4PRO with AffiPump · Reagent and reference-material QC
App noteDNA Hybridization & Aptamer Biosensor Development with SPRP4SPR
App noteUnderstanding the Mechanism of Action of Transmembrane ProteinsP4SPR · Protein–Small Molecule BindingPDF
App noteReversible Aptamer Sensing of a Small-Molecule DrugP4SPR · Therapeutic drug monitoring, aptamer design
App noteDetecting Proteins Directly from Whole BloodP4SPR · Point-of-care diagnostics, serologyBlog
BlogSurface Plasmon Resonance: Advantages and DisadvantagesMethods & Protocols · September 2026
BlogSPR vs ELISA: Differences and When to Use EachClinical & Diagnostics · September 2026
BlogLSPR vs SPR: Pros and ConsTechnology · September 2026
BlogA Day at Merck, and What We Keep HearingSPR on the Road · September 2026BlogFrom DNA Probes to Wearable Biosensors: How SPR Accelerates the PipelineBiosensing · April 2026
BlogReal-time monitoring of vancomycin using a split-aptamer surface plasmon resonance biosensorClinical & Diagnostics · November 2025BlogEasy SPR experiment set-up: the Affinité sensor differenceMethods & Protocols · April 2024BlogHow you can calibrate your SPR measurementMethods & Protocols · June 2022BlogIntegrating SPR into AAV Production PipelineDrug Discovery · June 2022BlogHow a true Portable SPR can revolutionize your R&D workflowMethods & Protocols · March 2022BlogEvaluation of a Novel Vaccine Platform by Using Portable SPRClinical & Diagnostics · January 2022BlogUsing Portable SPR to Speed Up Your Lateral Flow Assay DevelopmentMethods & Protocols · December 2021
BlogMeasuring Heparin Binding with SPR: The SARS-CoV-2 Spike StudyBiosensing · November 2021
BlogMeasuring Binding Affinity with SPR: Protein-Protein InteractionsBiosensing · November 2021BlogAffiCoat™ for Reducing Non-Specific Adsorption in SPR ExperimentsMethods & Protocols · September 2021BlogSPR to Examine Cross-Reactivity of Antibodies with SARS-CoV-2 Spike ProteinsClinical & Diagnostics · July 2021BlogSPR Platform Based on Ionic Liquids for Breast Cancer Biomarker DetectionClinical & Diagnostics · June 2021BlogPortable SPR for On-Site Testing of Environmental ContaminantsBiosensing · May 2021BlogSPR for Enriching Synthetic Biology ResearchBiosensing · May 2021BlogThe Ability of SPR Compared to BLI to Detect PSA in Human SerumClinical & Diagnostics · March 2021BlogDetection of Liposomes by Surface Plasmon ResonanceBiosensing · December 2020
BlogManual Injection vs Pump-Assisted SPR ExperimentTechnology · November 2020
BlogThe Advantages of a Portable SPR InstrumentMethods & Protocols · September 2020
BlogRapid Quality Control of Antibodies Using P4SPR™Biomanufacturing & QC · September 2020PDFNews
NewsYour Instrument. Fully Unleashed.Issue 6 · News · February 2026
NewsLensless SPR, Better Data, and a Transatlantic Adventure.Issue 5 · News · January 2026
NewsFrom Berkeley to Taipei — a year of SPR, everywhere.Issue 4 · News · December 2025
NewsTen years. Still making SPR make sense.Issue 2 · Case study · October 2025NewsSmall instruments. Big vision. Ten years of making SPR accessible.Issue 1 · News · September 2025Interactive tools
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ToolAre You Ready for SPR?Nine quick questions about your molecules, samples and goals. Get a readiness check, a suggested setup and sensor, and a checklist for your first expe ↗ToolTechnique Comparison ToolCompare SPR against 15 biophysical methods with an interactive heatmap and head-to-head comparison. ↗
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